Cancer rates could further increase by 50% to 15 million new cases in the year 2020,(WCR). One of worldwide problems is Colorectal cancer (CRC) with 1 million cases. Medicinal plants considered to be the most hopeful way for cancer treatment. The Cichorium endivia, L. plant materials were collected from Tanuma, Saudi Arabia. The anticancer activity of the plant root extract was examined on colon cancer cell line HCT-116. The extract degrees of activity were measured by determining cytotoxicity for the cell line compared with anticancer drug 5 FU (5-fluorouracil) for 24 hours. The IC50 was 0.78 ug/ml in case of the 5FU whereas 339 ug/ml in the plant extract. DNA cancer markers; P53 expression was the same in the control cells and the treated cells either by the plant root extract or the 5FU. Bcl2 showed low expression in the treated cells compared with the control but it was lower in cells treated with 5FU than the cells treated with root extract. TNF expression was the same in the cells treated with 5FU and the control one but the expression was so high in the cells treated with plant root extract.Interleukin IL-2, IL-4 showed low expression in cells treated with plant root extract compared with the 5FU. But in case of IL-6 cells treated with the plant root extract was so high compared with 5FU and the control cells. Thus, a combination of phenolic compounds, represents an enjoyable means of anticancer in CRC.